Effects of electric pulses on liver cancer cells: apoptosis induction and decrease of mitochondrial transmembrane potential / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 446-450, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-306541
Responsible library:
WPRO
ABSTRACT
In order to investigate the effects of electric pulses on cancer cells, we carried out the experiments with exposing HepG2 and L02 to electric pulses (1 kV/cm, l00 micros, 1 Hz) for different lengths of time (8 s, 15 s, 30 s, 60 s). Annexin V-FITC Kit and Flow cytometry were used to study the apoptosis of treated cells. The results showed that the electric pulses of 1 kV/cm, l00 micros, 1 Hz for 8 s could not induce tumor cells apoptosis. Apoptosis was observed when tumor cells were stimulated for 15 s and longer, and the apoptosis percentage increased with the increase of stimulation time. Furthermore, tumor cells were more sensitive than normal cells in response to electrical pulses. Rhodamine 123 and Laser Scanning Confocal Microscope (LSCM) were used to make a real-time study of mitochondrial transmembrane potential (Deltapsim) when the tumor cells were exposed to electric pulses for 60 s. No significant change of Deltapsim was observed within 30 s stimulation. After that, the Deltapsim increased sharply and declined later, suggesting that the mitochondrial pathway may be one of the apoptosis mechanism induced by electric pulses.
Full text:
Available
Health context:
Sustainable Health Agenda for the Americas
/
SDG3 - Health and Well-Being
/
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Goal 9: Noncommunicable diseases and mental health
/
Target 3.4: Reduce premature mortality due to noncommunicable diseases
/
Liver Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Radiation Effects
/
Time Factors
/
Apoptosis
/
Electromagnetic Fields
/
Membrane Potential, Mitochondrial
/
Hep G2 Cells
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2011
Document type:
Article