Multi-drug resistance and characteristic of integrons in Shigella spp. isolated from China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 56-61, 2011.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-306891
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp.</p><p><b>METHODS</b>Ninety Shigella strains (83 S. flexneri and 7 S. sonnei) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.</p><p><b>RESULTS</b>High prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%) carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intI3 was detected in the tested isolates. The prevalence of intI2 was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P<0.05). Gene cassettes dfrA17-aadA5, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.</p><p><b>CONCLUSION</b>The class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.</p>
Full text:
Available
Health context:
Sustainable Health Agenda for the Americas
/
Neglected Diseases
Health problem:
Goal 5: Medicines, vaccines and health technologies
/
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Shigella
/
Polymorphism, Restriction Fragment Length
/
Drug Resistance, Microbial
/
Base Sequence
/
China
/
Polymerase Chain Reaction
/
DNA Primers
/
Drug Resistance, Multiple
/
Integrons
/
Genetics
Type of study:
Prognostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2011
Document type:
Article