Association between the +491C/T polymorphism of beta2-adrenergic receptor gene and risk of essential hypertension in Kazakns of Xinjiang / 中华心血管病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigated the association between the +491C/T polymorphism of beta2-AR gene and risk of essential hypertension (EH) in Kazakns of Xinjiang.</p><p><b>METHODS</b>In this population-based case-control study, we recruited 507 Kazakns subjects (289 EH and 218 normotensives, aged from 30 - 60 years old) from the pastoral areas in Xinjiang. Peripheral blood were collected and the DNA was extracted by means of a standard phenol-chloroform method. The +491C/T genotypes of beta2-AR was identified by PCR-restriction fragment length polymorphism analysis. The distribution of genotypic and allelic frequencies between EH and controls were also compared.</p><p><b>RESULTS</b>The frequencies of the genotype CC, CT were 98.82% and 1.18%, respectively and there was no TT genotype in this population, the genotype distribution was consistent with Hardy-Weinberg equilibrium (chi2 = 0.018, P = 0.893). CC and CT genotypic frequencies were 97.92% and 2.08% respectively in EH, while there was no CT genotype in normotensives. The allelic frequencies of C, T were significantly different between EH (98.96%, 1.04%) and normotensives (100%, 0%, all P = 0.040). Systolic blood pressure and diastolic blood pressure were similar in subjects with various genotypes in the whole population (P > 0.05) but systolic blood pressure was significantly higher in males with CT genotype compared with males with CC genotype (P = 0.046).</p><p><b>CONCLUSION</b>The +491C/T polymorphism of beta2-AR gene is associated with essential hypertension and is a possible susceptible factor for essential hypertension in Kazakns of Xinjiang.</p>