Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae.</p><p><b>METHOD</b>The analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection.</p><p><b>RESULT</b>The calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively.</p><p><b>CONCLUSION</b>The contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions.</p>