Morphological changes in network of enteric nerve-interstitial cells of Cajal-smooth muscle cells in rats with multiple organ dysfunction syndrome and therapeutic effects of Dachengqi decoction (大承气汤) / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 422-429, 2010.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-308741
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Dachengqi Decoction (大承气汤, DCQD) on morphological changes in the network of enteric nerve-interstitial cells of Cajal (ICCs)-smooth muscle cells (SMC) of enteric deep muscular plexuses (DMP) in the rats with multiple organ dysfunction syndrome (MODS).</p><p><b>METHODS</b>One hundred Wistar rats of both sexes weighing 200 to 250 g were randomly divided into the control group, MODS group, and DCQD group. The morphologic changes of enteric nerve-ICC-SMC network in the DMP of intestine was observed using c-Kit and vesicular acetylcholine transporter/neuronal nitric oxide synthase immunohistochemical double-staining with whole-mount preparation technique, confocal laser scanning microscopy, and electron microscopy.</p><p><b>RESULTS</b>Compared with the control group, the distribution and densities of cholinergic/nitrergic nerves and ICC in the DMP (ICC-DMP) of intestine in the MODS group were significantly decreased (P<0.01), and the network of cholinergic nerve-ICC-SMC was disrupted; and the ultrastructural features of ICC-DMP, enteric nerve, and SMC were severely damaged. After treatment with DCQD, the damage in the network of enteric nerve-ICC-SMC was significantly recovered. Compared with the MODS group, the distribution and densities of cholinergic/nitrergic nerves and ICC-DMP in the DCQD group were significantly increased (P<0.01); and the ultrastructural features of ICC-DMP, enteric nerve, smooth muscle cells were significantly recovered.</p><p><b>CONCLUSIONS</b>DCQD can improve the gastrointestinal motility in MODS. The mechanism may be related to the effect of repairing the damages in the network of enteric nerve-ICC-SMC.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Plant Extracts
/
Microscopy, Confocal
/
Cell Biology
/
Therapeutic Uses
/
Interstitial Cells of Cajal
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Intestines
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Multiple Organ Failure
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2010
Document type:
Article