Diagnostic value for detection of aspergillus ribosomal RNA in fungal rhinosinusitis with hybridization in situ / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 852-855, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-309411
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a reliable, rapid assay for detecting pathogenic aspergillus species in fungal sinusitis.</p><p><b>METHODS</b>Thirty-seven formalin-fixed and paraffin-embedded surgical tissue specimens from patients with fungal sinusitis were used in the present study. The aspergillus specific oligonucleotide probe was designed, commercially synthesized, and digoxigenin-labeled. Twenty-three-base oligonucleotides was selected that was complementary to 18S ribosomal RNA sequences (18S-1 probe) for detecting medically important aspergillus species.</p><p><b>RESULTS</b>In situ hybridization for aspergillus rRNA was positive in 28 cases with the 18S-1 probe. Compared with HE (21) and methenamine-silver stain (23).</p><p><b>CONCLUSION</b>In situ hybridization provides rapid and accurate identification for fungal organism in tissues, and may be useful if cultures are negative or have not performed.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Aspergillosis
/
Aspergillus
/
Sinusitis
/
RNA, Fungal
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RNA, Ribosomal, 18S
/
In Situ Hybridization
/
Diagnosis
/
Genetics
/
Microbiology
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2007
Document type:
Article