Cortical neuron apoptosis induced by beta-amyloid peptide and protective effect of panoxadiol in mice / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 451-455, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-310432
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis of cortical neurons induced by beta-amyloid peptide (Abeta(1-40)) and the protective effect of panoxadiol.</p><p><b>METHODS</b>The Abeta(1-40) induced damage of primarily cultured mouse cortical neurons was examined with morphological observation, MTT assay, DNA agarose gel electrophoresis and Western-blot.</p><p><b>RESULT</b>After 48 h treated with 12 mumol/L Abeta(1-40), the cortical neurons showed apoptotic characteristics including decreased OD570 value in MTT assay, DNA cleavage fragment in electrophoresis and increased apoptotic cells. Western-blot showed that the expression of bcl-2 reduced significantly (P<0.05). Cell apoptosis was significantly attenuated in 40 mg/L panoxadiol treated group.</p><p><b>CONCLUSION</b>Panoxadiol can protect cultured cortical neurons from apoptosis induced by Abeta(1-40) in mice.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Pharmacology
/
Drugs, Chinese Herbal
/
Cells, Cultured
/
Cerebral Cortex
/
Amyloid beta-Peptides
/
Apoptosis
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Neuroprotective Agents
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Proto-Oncogene Proteins c-bcl-2
/
Cell Biology
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2008
Document type:
Article