ILs-HPLC simultanesous determination of five alkaloids in phellodenddri chinensis cortex / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3808-3812, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-310984
Responsible library:
WPRO
ABSTRACT
A RP-HPLC method was established for simultaneous determination of phellodendrine hydrochloride (PH1), magnoflorine hydrochloride (MH), jatrorrhizine hydrochloride (JH), palmatine hydrochloride (PH2) and berberine hydrochloride (BH) in Phellodendri Chinensis Cortex by using ionic liquids as mobile phase additives. The separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 μm) coupled with ultraviolet (UV) detection. The effect of extraction solvent, detection wavelength, length of alkyl chain on different imidazolium ionic liquids and concentration of ionic liquids on the separation and determination of alkaloids were investigated. Ionic liquid, [BMIm] BF4, can obviously improve the resolution and peak shape. This ILs-HPLC method is simple, rapid, and reliable, which can be used for determination of alkaloids in Phellodenddri Chinensis Cortex.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Phellodendron
/
Alkaloids
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Document type:
Article