The enhancing effect of electroporation and iontophoresis on the permeation of insulin through human skin / 药学学报

ABSTRACT

<p><b>AIM</b>To study the enhancing effect of electroporation and iontophoresis on the permeation of insulin through human cadaver skin in vitro.</p><p><b>METHODS</b>Using side by side two-chamber diffusion cells, the flux of insulin achieved with iontophoresis and electrophoration were compared.</p><p><b>RESULTS</b>The application of high-voltage pulse combined with iontophoresis resulted in higher flux transdermal permeation of insulin than either one technique alone (P < 0.05). Pulsing at a higher voltage increased the flux of insulin more dramatically than pulsing at a lower voltage (P < 0.01). The transdermal transport of insulin by 90 pulse of 500 V (exponential pulse generater, pulse time: 20-24 ms, pulse frequency: 3 pulse.min-1) followed by iontophoresis led to a quick input and a high steady flux.</p><p><b>CONCLUSION</b>Electroporation combined with iontophoresis can enhance the permeation of insulin significantly.</p>