The expression of platelet-derived growth factor-A and its receptor in acute radiation-induced skin ulcer in rats / 中华烧伤杂志
Chinese Journal of Burns
; (6): 359-362, 2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-312542
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the dynamic expression of platelet-derived growth factor-A (PDGF-A) and its receptor alpha (PDGFR-alpha) in different acute radiation-induced skin ulcers, and to explore the underlying mechanism involved in retarded healing of the ulcer.</p><p><b>METHODS</b>The model of acute radiation-induced skin ulcers in rats was replicated with 50 Gy 60Co gamma rays to the skin (radiation group, R, n = 55), rats with full - thickness skin excision wounds as control group (T, n = 55), and 5 normal rats to serve as normal control (NC) group. The expression of PDGF-A and PDGFR-alpha protein and PDGF-A mRNA was respectively assessed by means of histochemistry and in situ RT-PCR.</p><p><b>RESULTS</b>No PDGF-A expression was identified in the rat skin in NC group. The expression of PDGF-A and PDGFR were reduced in R group during inflammatory responsive and granulation formation periods (14 - 28 days after radiation, the IA value of PDGF-A varied from 14.0 +/- 1.2 to 20.3 +/- 1.2 compared with that in T group in which the IA value of PDGF-A at the same period (3 - 9 days after injury) varied from 20.0 +/- 1.6 to 28.3 +/- 1.0, and reduced gradually during scar formation period (55 days after radiation).</p><p><b>CONCLUSION</b>The reduction of PDGF-A and PDGFR-expression may be partially involved in the mechanism of retarded healing of acute radiation-induced skin ulcers.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Radiation Injuries, Experimental
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Skin Ulcer
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Wound Healing
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Platelet-Derived Growth Factor
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Rats, Wistar
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Receptor, Platelet-Derived Growth Factor alpha
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Gamma Rays
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Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2005
Document type:
Article