Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 165-170, 2005.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-316358
Responsible library:
WPRO
ABSTRACT
Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high salinity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediation. In order to characterize microbial diversity and look for clues on the relationship between microbial community structure and hydrochemistry, a culture-independent examination of a typical groundwater sample obtained from a seashore landfill was conducted by sequence analysis of 16S rDNA clone library. Two sets of universal 16S rDNA primers were used to amplify DNA extracted from the groundwater so that problems arising from primer efficiency and specificity could be reduced. Of 74 clones randomly selected from the libraries, 30 contained unique sequences whose analysis showed that the majority of them belonged to bacteria (95.9%), with Proteobacteria (63.5%) being the dominant division. One archaeal sequence and one eukaryotic sequence were found as well. Bacterial sequences belonging to the following phylogenic groups were identified Bacteroidetes (20.3%), beta, gamma, delta and epsilon-subdivisions of Proteobacteria (47.3%, 9.5%, 5.4% and 1.3%, respectively), Firmicutes (1.4%), Actinobacteria (2.7%), Cyanobacteria (2.7%). The percentages of Proteobacteria and Bacteroides in seawater were greater than those in the groundwater from a non-seashore landfill, indicating a possible influence of seawater. Quite a few sequences had close relatives in marine or hypersaline environments. Many sequences showed affiliations with microbes involved in anaerobic fermentation. The remarkable abundance of sequences related to (per)chlorate-reducing bacteria (ClRB) in the groundwater was significant and worthy of further study.
Full text:
Available
Health context:
SDG3 - Target 3.9 Reduce the amount of deaths produced by dangerous chemicals and the pollution of the air, water and soil
/
SDG3 - Health and Well-Being
Health problem:
Water, Sanitation and Hygiene
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Target 3.9: Reduce the amount of deaths produced by dangerous chemicals and the pollution of the air, water and soil
Database:
WPRIM (Western Pacific)
Main subject:
Seawater
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Sewage
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Bacteria
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Water Microbiology
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Water Pollutants
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RNA, Ribosomal, 16S
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Environmental Monitoring
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Polymerase Chain Reaction
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Genetics
/
Methods
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2005
Document type:
Article