Rho-associated coiled kinase inhibitor Y-27632 promotes neuronal-like differentiation of adult human adipose tissue-derived stem cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 3332-3335, 2012.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-316514
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Y-27632 is a specific inhibitor of Rho-associated coiled kinase (ROCK) and has been shown to promote the survival and induce the differentiation of a variety of cells types. However, the effects of Y-27632 on adult human adipose tissue-derived stem cells (ADSCs) are unclear. This study aimed to investigate the effects of Y-27632 on the neuronal-like differentiation of ADSCs.</p><p><b>METHODS</b>ADSCs were isolated from women undergoing plastic surgery and cultured. ADSCs were treated with different doses of Y-27632 and observed morphological changes under microscope. The expression of nestin, neuron specific enolase (NSE) and microtubule-associated protein-2 (MAP-2) in ADSCs treated with Y-27632 was detected by immunocytochemistry and Western blotting analysis.</p><p><b>RESULTS</b>Y-27632 had the potency to induce neuronal-like differentiation in ADSCs in a dose-dependent manner. Moreover, the differentiation induced by Y-27632 was recovered upon drug withdraw. ADSCs treated with Y-27632 expressed neuronal markers such as NSE, MAP-2 and nestin while untreated ADSCs did not express these markers.</p><p><b>CONCLUSION</b>Selective ROCK inhibitor Y-27632 could potentiate the neuronal-like differentiation of ADSCs, suggesting that Y-27632 could be utilized to induce the differentiation of ADSCs to neurons and facilitate the clinical application of ADSCs in tissue engineering.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pyridines
/
Stem Cells
/
Cell Differentiation
/
Cells, Cultured
/
Adipose Tissue
/
Cell Biology
/
Amides
/
Neurons
Limits:
Adult
/
Female
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2012
Document type:
Article