Interleukin-1β inhibits collagen synthesis and promotes its decomposition in cultured cardiac fibroblasts / 生理学报
Acta Physiologica Sinica
; (6): 355-361, 2008.
Article
in En
| WPRIM
| ID: wpr-316719
Responsible library:
WPRO
ABSTRACT
The present study aimed to investigate the effects of interleukin-1β (IL-1β) at different doses on collagen synthesis and decomposition in cultured cardiac fibroblasts from neonatal Sprague-Dawley rat. Cardiac fibroblasts were treated with IL-1β (0.01, 0.1, 1, 10, 100 ng/mL) for 24 h. Cell DNA synthesis was measured by (3)H-thymidine ((3)H-TdR) incorporation and collagen synthesis was measured by (3)H-proline ((3)H-Pro) incorporation. Matrix metalloproteinase (MMP) activity was measured by gelatinase zymography. MMP-2, MMP-9 protein expressions were measured by Western blot. mRNA expressions of MMP-2 and MMP-9 were detected by reverse transcription-polymerase chain reaction (RT-PCR). Compared with that in the control group, the incorporation of (3)H-TdR and (3)H-Pro decreased in high-dose IL-1β groups (≥0.1 ng/mL) but not in low-dose IL-1β group (0.01 ng/mL). IL-1β significantly increased MMP-2 and MMP-9 activities. IL-1β (0.01-100 ng/mL) also dose-dependently increased the protein and mRNA expressions of MMP-2 and MMP-9 (P<0.05, P<0.01), respectively. These results suggest that IL-1β decreases collagen synthesis and MMP activities through transcriptional and posttranslational processes via degrading collagen in a dose-dependent way. Elevation of IL-1β is possibly involved in the process of ventricular remodeling after myocardial infarction, and the concentration of IL-1β is possibly a major factor which affects the extent of ventricular remodeling.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Cells, Cultured
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Collagen
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Rats, Sprague-Dawley
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Ventricular Remodeling
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9
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Cell Biology
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Interleukin-1beta
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Fibroblasts
Limits:
Animals
Language:
En
Journal:
Acta Physiologica Sinica
Year:
2008
Document type:
Article