Effects of diazoxide on Fas/FasL protein expressions in rat myocardium suffered from long-term hypothermic preservation / 生理学报
Acta Physiologica Sinica
; (6): 11-16, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-316767
Responsible library:
WPRO
ABSTRACT
The purpose of this study was to investigate the effect of a mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) opener, diazoxide (DE), on Fas/FasL protein expressions in rat heart suffered from long-term hypothermic preservation. The Langendorff isolated rat heart model was used. The hearts were stored in 4 °C Celsior solution with or without (control) DE for 8 h followed by 60 min of reperfusion. The recovery of rate-pressure product (RPP) was observed. Apoptotic cardiomyocytes were detected by TdT-mediated dUTP nick end labeling (TUNEL) technique. The expressions of Fas/FasL proteins were also analyzed by immunohistochemical method. The results showed that compared with the control group, DE (30 mmol/L) increased the recovery of RPP during reperfusion, reduced the percentage of apoptotic cells and the expressions of Fas and FasL proteins in rat hearts suffered from 8 h of hypothermic preservation. The above effects of DE were attenuated by a mitoK(ATP) channel inhibitor 5-hydroxydecanoate (5-HD). These results indicate that DE could alleviate rat myocardial injury induced by ischemia-reperfusion through reducing the expressions of Fas and FasL proteins via opening of mitoK(ATP)channel.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Potassium Channels
/
Cryopreservation
/
Apoptosis
/
Fas Receptor
/
Cell Biology
/
Potassium Channel Blockers
/
Myocytes, Cardiac
/
Decanoic Acids
/
Diazoxide
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2008
Document type:
Article