Compatibility principle research of Yupingfeng powder based on cocktail probe substrates approaches / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 618-621, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-318647
Responsible library:
WPRO
ABSTRACT
To elucidate the compatibility principle of Yupingfeng powder, a cocktail probe substrates approaches were developed. The enzymatic activity of cytochrome P450 from rat liver microsome was evaluated after being interfered with 7 prescriptions of Yupingfeng powder, which was designed according to the decomposed recipes design of traditional Chinese medicine. In vitro test, rat liver microsome incubation system was utilized to detect the 50% inhibitory concentrations of Yupingfeng powder with the decomposed recipes to cytochrome P450 (CYP1A2, CYP2A6 ,CYP2C9, CYP2C19, CYP3A4) enzyme. The CYPs IC50 value of Yupingfeng powder with different compatibility were greater than crude drug 1.0 g x L(-1), which indicated that all Yupingfeng powder prescriptions had no significant inhibitory activity to cytochrome P450. For CYP1A2, CYP2C19, CYP3A4, the IC50 value of Yupingfeng powder with the decomposed recipes had a tendency to increase, compared with the major impact factor from Yupingfeng powder. For CYPs, the detected IC50 of Yupingfeng powder with the decomposed recipes tended to decrease, compared with the linearly predicted value. From the point of view of the impact of drugs on the metabolic activity, the compatibility of Yupingfeng powder has certain advantages and reasonable.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Powders
/
Materials Testing
/
Microsomes, Liver
/
Drugs, Chinese Herbal
/
Inhibitory Concentration 50
/
Cytochrome P-450 Enzyme System
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Document type:
Article