HPLC-MS/MS for determination of astragalin in rat plasma and its pharmacokinetics / 南方医科大学学报
Journal of Southern Medical University
; (12): 1049-1052, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-319481
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a LC-MS/MS method for determination of astragalin in rat plasma and study its pharmacokinetics in rats.</p><p><b>METHODS</b>The analytical column was packed with ZORBAX SB-C18, and the mobile phase was methanol-water containing 10 mmol/L ammonium acetate-formic acid (80200.15, V/V/V). Quercetin was used as the internal standard (IS). Multiple reaction monitoring (MRM) mode was employed, and the transition of m/z was m/z 449.1→m/z 287.1 for astragalin, and m/z 301.1→m/z 151.1 for IS.</p><p><b>RESULTS</b>A good liner relationship was obtained within the range of 1.00-1000 ng/ml (r(2)=0.9929), and the lower limit of quantification of astragalin was 1.00 ng/ml in rat plasma. The extraction recoveries were all above 93%. After oral administration of astragalin, the maximum plasma concentration of astragalin was 231.1∓67.3 ng/ml and the time to reach this value was 0.5∓0.1 h, with a half-life of 3.9∓1.3 h and an AUC of 782.6∓152.8 ng·h/ml.</p><p><b>CONCLUSION</b>The method is highly sensitive, selective and rapid for determination of the concentration of astragalin in rat plasma to facilitates the study of its pharmacokinetics.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Blood
/
Pharmacokinetics
/
Chromatography, High Pressure Liquid
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Rats, Sprague-Dawley
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Kaempferols
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Tandem Mass Spectrometry
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Document type:
Article