Promoter methylation of Wilms' tumor gene on the X- chromosome in gastric cancer / 南方医科大学学报
Journal of Southern Medical University
; (12): 318-321, 2013.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-322054
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in methylation levels of the promoters of the tumor suppressor gene Wilms' tumor gene on the X-chromosome (WTX) and its possible role in gastric cancer.</p><p><b>METHODS</b>WTX promoter methylation levels were detected in 20 pairs of specimens of gastric cancer and matched normal tissues and in 3 gastric cancer cell lines (MGC803, SCG7901, and BGC823) using the Sequenom MassARRAY quantitative analysis system. The gastric cancer cell line BGC823 was treated with 5-aza-2'-deoxycytidine (5-aza-dC) for demethylation and the changes in the level of WTX promoter methylation were investigated.</p><p><b>RESULTS</b>WTX promoter methylation levels were very low and showed no significant differences among normal gastric tissues, gastric cancer tissues and the 3 gastric cancer cell lines. In BGC823 cells, treatment with 5-aza-dC did not obviously affect the promoter methylation levels of WTX.</p><p><b>CONCLUSION</b>High methylation levels of WTX promoters are rare in gastric cancer.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Stomach Neoplasms
/
Genes, Wilms Tumor
/
Promoter Regions, Genetic
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DNA Methylation
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Chromosomes, Human, X
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Cell Line, Tumor
/
Genetics
/
Metabolism
Limits:
Humans
Language:
English
Journal:
Journal of Southern Medical University
Year:
2013
Document type:
Article