Application of two-dimensional electrophoresis and mass spectrometry in the research of testicular proteins / 中华男科学杂志
National Journal of Andrology
; (12): 85-89, 2003.
Article
in Zh
| WPRIM
| ID: wpr-322543
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To evaluate the application of two-dimensional electrophoresis and mass spectrometry in the research of proteins expressed in testis.</p><p><b>METHODS</b>Protein from adult ICR mouse testes was extracted by two-dimensional electrophoresis. Two protein spots were cut from the gel, then the proteins were digested in-gel by enzyme and the generated peptides were measured by matrix assisted laser desorption/ionization-time of flight mass spectrometry. The proteins were identified through database searching.</p><p><b>RESULTS</b>Two spots in Commasie Brilliant Blue-stained gel were identified as serum albumin and protein disulfide isomerase by database searching.</p><p><b>CONCLUSIONS</b>This rapid high resolution and efficient method is a very powerful way to analyze testicular proteins.</p>
Full text:
1
Database:
WPRIM
Main subject:
Mass Spectrometry
/
Testis
/
Electrophoresis, Gel, Two-Dimensional
/
Proteins
/
Chemistry
/
Mice, Inbred ICR
Limits:
Animals
Language:
Zh
Journal:
National Journal of Andrology
Year:
2003
Document type:
Article