Effects of AMPK on the transcriptional activity of FOXO1 and ubiquitin ligase MuRF1 expression in rat cardiomyocytes / 南方医科大学学报
Journal of Southern Medical University
; (12): 2419-2422, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-323646
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of AICAR on the activity of transcription factor FOXO1 and expression of ubiquitin ligase MuRF1 in rat cardiomyocytes, and explore the possible role of AMP-activated protein kinase (AMPK) in proteolysis pathways.</p><p><b>METHODS</b>In vitro cultured neonatal rat cardiac myocytes were treated with AICAR, and Western blotting was used to detect the phosphorylation of FOXO1 and expression of MuRF1 in the cells.</p><p><b>RESULTS</b>AICAR activated AMPK in rat cardiac myocytes. Activated AMPK significantly inhibited the phosphorylation of FOXO1 and increased MuRF1 protein expression.</p><p><b>CONCLUSION</b>AMPK may regulate proteolysis by activating FOXO1 transcription factor and up-regulating MuRF1 expression.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Ribonucleotides
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Cells, Cultured
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Rats, Sprague-Dawley
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Myocytes, Cardiac
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Ubiquitin-Protein Ligases
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Forkhead Transcription Factors
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AMP-Activated Protein Kinases
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Tripartite Motif Proteins
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Aminoimidazole Carboxamide
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Document type:
Article