DNA methylation levels of genome in cFb transdifferentiation induced by SiO2 in rats / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 326-330, 2012.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-324270
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the DNA methylation levels of genome in cFb transdifferentiation induced by SiO2 in rats.</p><p><b>METHODS</b>The primary macrophages and fibrocytes of SD rats were co-cultured directly and indirectly, which were exposed to SiO2 at the doses of 25, 50 and 100 g/ml. The transdifferentiation of cFb was identified with immunohistochemical assay. The genomic DNA methylation levels of cFb were detected with HPLC.</p><p><b>RESULTS</b>Under the condition of indirect co-culture, as compared with control group, the genomic DNA methylation levels of cFb exposed to SiO2 at the doses of 25, 50 and 100 g/ml reduced by 19.9%, 26.9% and 30.3%, respectively (P < 0.05); as compared with cFb exposed to 100 g/ml SiO2, the genomic DNA methylation levels of cFb exposed to 5-aza-dC decreased by 22.0% (P < 0.05). Under the condition of ThinCert(TM) direct co-culture, as compared with control group, the genomic DNA methylation levels of cFb exposed to SiO2 at the doses of 25, 50 and 100 g/ml reduced by 22.2%, 30.2% and 36.7%, respectively (P < 0.05); as compared with cFb exposed to 100 g/ml SiO2, the genomic DNA methylation levels of cFb exposed to 5-aza-dC decreased by 20.6% (P < 0.05).</p><p><b>CONCLUSION</b>Under the co-culture condition in vitro, SiO2 could reduce the genomic DNA methylation levels of cFb. The ThinCert(TM) direct co-culture can be used to study the silicosis fibrosis.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Genome
/
Rats, Sprague-Dawley
/
Silicon Dioxide
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Coculture Techniques
/
DNA Methylation
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Cell Biology
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Cell Transdifferentiation
/
Fibroblasts
/
Lung
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2012
Document type:
Article