Quality evaluation of Flos Carthami / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2642-2646, 2008.
Article
in Zh
| WPRIM
| ID: wpr-324834
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop methods for qualitative and quantitative analyses of Flos Cartnami from three aspects, pigments, flavonoids and adenosine.</p><p><b>METHOD</b>A method using HPLC coupled with electrochemical detector was developed to determine the content of hydroxysafflor yellow A and fingerprint of Flos Carthami. The chromatographic separation was performed on a Zorbax SB C18 column (4.6 mm x 250 mm, 5 microm) by gradient elution with phosphate buffer and acetonitrile at a flow-rate of 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the working electrode was glassy carbon, the counter electrode was Pt, and the applied potential was + 800 mV. Concentration of adenosine was determined by HPLC-UV on an Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) with water-acetonitrile (95:5) as mobile phase, the flow rate was 1.0 mL x min(-1), the column temperature was 40 degrees C and the detection wavelength was 260 nm. The content of cartharmin was detected using a spectrophotometric method.</p><p><b>RESULT</b>Twenty-one common chromatographic peaks were selected as characteristic peaks in the chromatogram of sample solution of Flos Cartnami. Seven peaks were identified as hydroxysafflor yellow A, 6-hydroxykaempferol-3-O-glucoside, rutin, quercetin-3-O-glucoside, kaempferol-3-O-rutinoside, quercetin, kaempferol. The contents of hydroxysafflor yellow A and adenosine were from 0.35% to 3.58% and from 0.03% per hundred to 0.49% per hundred, respectively.</p><p><b>CONCLUSION</b>The methods can be used to evaluate the quality of Flos Carthami.</p>
Full text:
1
Database:
WPRIM
Main subject:
Plants, Medicinal
/
Quinones
/
Flavonoids
/
Adenosine
/
Chalcone
/
Chemistry
/
Chromatography, High Pressure Liquid
Type of study:
Prognostic_studies
/
Qualitative_research
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Document type:
Article