Analysis of genetic diversity of wild Rehmannia glutinosa by using RAPD and ISSR markers / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the genetic diversity of wild Rehmannia glutinosa and evaluate and compare random amplified polymorphic DNA (RAPD) and inter sample sequence repeat (ISSR) for analysis of R. glutinosa accessions.</p><p><b>METHOD</b>Two molecular markers, RAPD and ISSR were used for analyzing 55 wild R. glutinosa accessions.</p><p><b>RESULT</b>Average 16.00 and 19.08 bands were amplified by RAPD primers and ISSR primers respectively, and the percentage of polymorphic bands were 89.58% and 94.32% respectively; Fifty-five R. glutinosa accessions categorized into 7 clusters were identified by unweighted pair-group method, arithmetic average (UPGMA) method.</p><p><b>CONCLUSION</b>A high level of genetic diversity of wild Rehmannia glutinosa was displayed at DNA level, and genetic diversity coefficient of R. glutinosa from different production areas was 0.63-0.98, and ISSR marker can detect higher genetic diversity of R. glutinosa germplasms than RAPD marker.</p>