Screening of proteins binding to FXR1P using yeast two-hybrid technique / 南方医科大学学报
Journal of Southern Medical University
; (12): 2394-2400, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-325108
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the proteins interacting with FXR1P for functional investigation of FXR1P.</p><p><b>METHODS</b>The yeast strain AH109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain cDNA library. The positive clones were screened and identified by sequence analysis.</p><p><b>RESULTS</b>The recombinant expression vector pGBKT7/FXR1 was constructed successfully. Five proteins binding to FXR1P were screened from human fetal brain cDNA library using the yeast two-hybrid system, including CMAS, FTH1, GOLGA4, HSD17B1 and CSH1.</p><p><b>CONCLUSIONS</b>These results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Autoantigens
/
Gene Library
/
RNA-Binding Proteins
/
Two-Hybrid System Techniques
/
Estradiol Dehydrogenases
/
Protein Interaction Domains and Motifs
/
Ferritins
/
Genetics
/
Membrane Proteins
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Document type:
Article