Isolation and characterization of H2-producing strains Enterobacter sp. and Clostridium sp / 生物工程学报
Chinese Journal of Biotechnology
; (12): 152-156, 2007.
Article
in Zh
| WPRIM
| ID: wpr-325402
Responsible library:
WPRO
ABSTRACT
Two hydrogen-producing bacterial strains were newly isolated and identified as Enterobacter sp. Z-16 and Clostridium sp. C-32 by 16S rDNA sequence analysis. Various parameters for hydrogen production, including substrates, initial pH and temperature, have been studied. The optimum condition for hydrogen production of strain Z-16 were achieved as: initial pH7.0, temperature 35 degrees C , sucrose as the favorite substrate. In comparison, The optimum condition for hydrogen production of strain C-32 were obtained as: initial pH8.0, temperature 35 degrees C , maltose as the favorite substrate . Under batch fermentative hydrogen production conditions, the maximal hydrogen conversion rate for strain Z-16 and strain C-32 were 2.68 mol H2/mol sucrose and 2.71mol H2/mol maltose, respectively. Using glucose as substrate, the hydrogen conversion rate of strain Z-16 and strain C-32 were 2.35 and 2.48 mol H2/mol glucose, respectively. This research suggest a good application potential of strain Z-16 and C-32 in the future biological hydrogen production.
Full text:
1
Database:
WPRIM
Main subject:
Species Specificity
/
Sucrose
/
Temperature
/
RNA, Ribosomal, 16S
/
Polymerase Chain Reaction
/
Clostridium
/
Microscopy, Electron, Transmission
/
Enterobacter
/
Fermentation
/
Genetics
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2007
Document type:
Article