Comparative study on in vitro stationary culture system or superfusion culture system of cow mammary tissue / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1040-1046, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-325428
Responsible library:
WPRO
ABSTRACT
Dairy cow mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation. The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 12-60 h in DMEM plus 10% calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within 60-108 h. Both culture systems had some advantages and disadvantages.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Perfusion
/
Time Factors
/
Kinetics
/
Microscopy, Electron
/
Bioreactors
/
Tissue Culture Techniques
/
Mammary Glands, Animal
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Document type:
Article
