Research of ITK regulation on mouse spleen lymphocytes proliferation and differentiation / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 269-271, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-325570
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>By using the RNAi method to inhibit Itk protein expression specificity, to observe lymphocytes proliferation and cytokines production, verify its function as a drug target.</p><p><b>METHODS</b>Designed siRNA aims at Itk sequence according to its sequence and solid structure, then electrotransfected into mouse spleen lymphocytes, We validated the decrease of Itk protein by Western-Blot, and detected the change of the cell proliferation by MTS and the change of inflammatory cytokines by ELISA.</p><p><b>RESULTS</b>Itk protein can be suppressed by Itk-siRNA, there were significantly reduced compared to its control group on cell proliferation as well as cytokine secretion such as IL-2, IL-4, IL-5, IFN-gamma. They all have statistical difference (P < 0.05).</p><p><b>CONCLUSION</b>Itk has an important immunomodulatory effect in mouse spleen lymphocytes proliferation and secretion of inflammatory cytokines.This can supply an experimental basis to regard Itk as drug target for inflammation therapy.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Spleen
/
Protein-Tyrosine Kinases
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Lymphocytes
/
Cell Differentiation
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Cytokines
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Cell Biology
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Cell Proliferation
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Allergy and Immunology
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Genetics
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Mice, Inbred BALB C
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Document type:
Article