Identification of genes regulated by HBV preS1-transactivated protein 2 binding protein 1 / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify genes regulated by HBV preS1-transactivated protein 2 binding protein 1 (PS1TP2BP1).</p><p><b>METHODS</b>PS1TP2BP1 gene was amplified by polymerase chain reaction (PCR) technique and cloned into the eukaryotic expression vector pcDNA 3.1/my-c-His A. The mRNAs isolated from HepG2 cells transfected recombinant eukaryotic expression vector pcDNA 3.1/myc-HisA-PS1TP2BP1 and pcDNA 3.1/myc-HisA empty vector were used to construct subtractive library. The differentially expressed genes were identified and analyzed.</p><p><b>RESULTS</b>35 differentially expressed clones were obtained. Colony PCR identified 15 clones with 200-1000 bp inserts. Sequence analysis identified 15 differentially expressed genes.</p><p><b>CONCLUSION</b>This study provides data for further characterize the function of PS1TP2BP1.</p>