Expression and activity analysis of human 67kD laminin receptor in Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology
; (12): 602-606, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-327979
Responsible library:
WPRO
ABSTRACT
To carry out the secretive expression of human 67 kD laminin receptor (67LR), recombinant expression plasmid pPIC9K-67LR was constructed by inserting of 67LR cDNA into yeast expression vector pPIC9K. The 67LR protein was expressed in Pichia pastoris after induced by methanol, and about 12.56 mg electrophoresis purity 67LR could be obtained after the purification of 1L culture using affinity chromatograph column. In vitro competitive binding assay showed that target protein has an excellent biological activity. The successful expression of 67LR has placed a solid foundation for the research on structure and functions of 67LR.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Plasmids
/
Recombinant Proteins
/
Receptors, Laminin
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Document type:
Article