Cloning and expression of a new glucoamylase gene / 生物工程学报
Chinese Journal of Biotechnology
; (12): 477-524, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-328001
Responsible library:
WPRO
ABSTRACT
According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Rhizopus
/
Recombinant Proteins
/
Fungal Proteins
/
Molecular Sequence Data
/
Gene Expression Regulation, Enzymologic
/
Gene Expression Regulation, Fungal
/
Blotting, Western
/
Glucan 1,4-alpha-Glucosidase
/
Cloning, Molecular
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Document type:
Article