Determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnikovia divaricata and HPLC fingerprint analysis / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1731-1734, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-328070
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop HPLC methods for the determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnicovia divaricata and of HPLC fingerprint to compare the wild and culture varieties.</p><p><b>METHOD</b>Conditions of determination Shimadzu C18 column (4.6 mm x 150 mm, 5 microm), methanol-water (4060) as mobile phase with the flow rate of 1 mL x min(-1). The detection wavelength was 254 nm. Conditions of HPLC fingerprint MG II C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-water with the flow rate of 1 mL x min(-1), using linear gradient elution, the column temperature was 30 degrees C.</p><p><b>RESULT</b>The average recovery of prim-O-glucosylcimifugin was 99.6% (RSD 0.72%, n=6). The average recovery of 5-O-methylvisammisoide was 102.6% (RSD 0.88%, n=6). The contents of prim-o-glucosylcimifugin in wild and culture varieties were (4.96 +/- 2.59) and (3.61 +/- 1.82) mg x g(-1) respectively. The contents of 5-O-methylvisammisoide were (3.91 +/- 2.09) and (4.37 +/- 2.02) mg x g(-1) respectively. The compositions of S. divaricata were effective separated under the conditions of HPLC fingerprint.</p><p><b>CONCLUSION</b>The HPLC determination method of prim-O-glucosylcimifugin and 5-O-methylvisammisoide is convenient and accurate. The HPLC fingerprint analysis method could be a basis for quality control and classification evaluate of S. divaricata.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Quality Control
/
Xanthenes
/
Drugs, Chinese Herbal
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Chemistry
/
Chromatography, High Pressure Liquid
/
Apiaceae
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Methods
/
Monosaccharides
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article