Development of salivary gland tumors in pleomorphic adenoma gene 1 transgenic mice / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 390-395, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-329452
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Activation and overexpression of pleomorphic adenoma (PLAG1) gene due to t(3;8)(p21;q12) translocation are associated with the development of human pleomorphic adenomas of the salivary glands. This study was conducted to generate ubiquitously-expressed or tissue-specific expressed PLAG1 transgenic mice and to elucidate the role of PLAG1 gene in tumorigenesis in vivo.</p><p><b>METHODS</b>Human PLAG1 cDNA was cloned from salivary gland tumor or placenta tissues by RT-PCR. Ubiquitous expression vector pCMV-EGFP/PLAG1 driven by CMV promoter and tissue-specific expression vector pMMTV-PLAG1 driven by MMTV LTR were constructed. NIH3T3 cells transiently transfected with pCMV-EGFP/PLAG1 showed high expression of PLAG1 in nucleus. Transgenes were microinjected into pronucleus of zygotes to generate transgenic mice.</p><p><b>RESULTS</b>It was found that the human PLAG1 cDNA cloned from several salivary gland tumor and normal placenta tissues consistently showed a variation of a single nucleotide at the same position when compared with the human PLAG1 cDNA sequence in Genbank (Accession No. U65002), which led to T458P at protein level. It might be a single nucleotide polymorphism (SNP)locus. Fused EGFP/PLAG1 protein was found to be localized in the nucleus of NIH3T3 cells transiently transfected with pCMV-EGFP/ PLAG1. Several pCMV-EGFP/PLAG1 and pMMTV-PLAG1 transgenic mouse lines were obtained respectively. As might be expected, pMMTV-PLAG1 transgenic mice spontaneously developed salivary gland tumors in three independent lines, among which, line 42 showed tumorigenic phenotype in 100% of transgenic mice within three months after birth.</p><p><b>CONCLUSION</b>Overexpression of PLAG1 gene plays a crucial role in tumorigenesis of salivary gland tumors.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Plasmids
/
Recombinant Fusion Proteins
/
Salivary Gland Neoplasms
/
Mice, Transgenic
/
Molecular Sequence Data
/
Base Sequence
/
Transfection
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NIH 3T3 Cells
/
Green Fluorescent Proteins
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2003
Document type:
Article