Differentiation of rat neural stem cells and its relationship with environment / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 1-7, 2004.
Article
in En
| WPRIM
| ID: wpr-329661
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the differentiation fates of rat neural stem cells (NSCs) in different environmental conditions.</p><p><b>METHODS</b>NSCs derived from 16-day-old rat embryo were proliferated in vitro and implanted into the brain of rats with intra-cerebral hemorrhage. At the same time some NSCs were co-cultured in vitro with Schwann cells derived from newborn rats. MAP-2, GFAP and GalC (which are the specific markers of neural cells, astrocytes and oligodendrocytes respectively), BrdU and beta-tubulin were detected by immunohistochemical and immunofluorescent methods.</p><p><b>RESULTS</b>BrdU positive cells that were implanted into the brain distributed around the hemorrhagic area. The majority of them were GFAP positive astrocytes while a few of them were beta-tubulin positive neural cells or GalC positive oligodendrocytes. After being co-cultured with Schwann cells in vitro, NSCs are predominately shown beta-tubulin and MAP-2 positive, and only a minority of them were GFAP or GalC positive.</p><p><b>CONCLUSIONS</b>The hemorrhagic environment in vivo induces NSCs to differentiate mainly into astrocytes while co-culture with Schwann cells in vitro induce the majority of NSCs to differentiate into neural cells.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Physiology
/
Schwann Cells
/
Sciatic Nerve
/
Stem Cells
/
General Surgery
/
Caudate Nucleus
/
Cell Differentiation
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Cell Movement
/
Cells, Cultured
Limits:
Animals
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2004
Document type:
Article