The role of p38 on the differentiation of MSCs to myoblasts / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 345-348, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-330099
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>Inducing mesenchymal stem cells (MSCs) differentiate to myoblasts with 5-azacytidine(5-Aza-CR), investigating the expression of Myf5 and the role of the signal transduction case of p38 in all the course of differentiation.</p><p><b>METHODS</b>Separating and purifying bone marrow-derived MSCs, inducing MSCs differentiation to myoblasts with 10 micromol/L 5-Aza-CR, assaying the gene expression time of Myf5 with RT-PCR method, the antigen expression of myosin with immunohistochemistry method and observing the changes of the activity of phosphorylation p38 before and after inhibited by SB203580 with Western-blot method.</p><p><b>RESULTS</b>MSCs begin to express Myf5 delayed to the 9th day after inhibited by SB203580. Some of MSCs express myosin at the 7th day after induced; The phosphorylation p38 activity of MSCs enhanced after induced by 5-Aza-CR but obviously decreased after inhibited by SB203580.</p><p><b>CONCLUSION</b>MSCs can express myogenic regulator factors and orientation differentiate to myoblasts after induced by 5-Aza-CR, p38 really have a positive signal transduction affection in this course.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Azacitidine
/
Bone Marrow Cells
/
Signal Transduction
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Cell Differentiation
/
Cells, Cultured
/
Cell Biology
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Myoblasts
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P38 Mitogen-Activated Protein Kinases
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Myogenic Regulatory Factor 5
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Mesenchymal Stem Cells
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2004
Document type:
Article