Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2208-2211, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-330319
Responsible library:
WPRO
ABSTRACT
In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Quality Control
/
Scorpions
/
Molecular Sequence Data
/
Drug Contamination
/
Classification
/
Electron Transport Complex IV
/
DNA Barcoding, Taxonomic
/
Arthropod Proteins
/
Genetics
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Document type:
Article