Anti-tumor effect of anti-dsDNA autoantibodies / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 73-76, 2005.
Article
in Zh
| WPRIM
| ID: wpr-331225
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effects of anti-dsDNA autoantibodies on growth of tumor in vitro and in vivo.</p><p><b>METHODS</b>BALB/c mice were inoculated with inactivated tumor cells and challenged s.c. with SP 2/0 and Wehi 164 tumor cells four weeks after the last inoculation. The naïve mice were inoculated with SP 2/0 tumor cells immediately after incubating with sera derived from the immunized mice at week 6. Then the tumor size was examined. In vitro, the cytotoxicity of anti-dsDNA autoantibodies to tumor cells was analysed. Furthermore, apoptosis of SP 2/0 and Wehi 164 tumor cells induced by anti-dsDNA autoantibodies was examined by FACS.</p><p><b>RESULTS</b>In vivo study showed that the growth of SP 2/0 and Wehi 164 tumors were inhibited in mice with anti-dsDNA autoantibodies, but not in mice lack of anti-dsDNA autoantibodies. In vitro, apoptosis of SP 2/0 and Wehi 164 tumor cells was induced when the tumor cells were incubated with the sera containing anti-dsDNA autoantibodies. Statistical analysis showed that the ability of anti-dsDNA autoantibodies to induce apoptosis of SP 2/0 and Wehi 164 tumor cells was significantly correlated with affinity (r = 0.990, P < 0.01 and r = 0.901, P < 0.05).</p><p><b>CONCLUSION</b>Anti-dsDNA autoantibodies have inhibitory effect on tumor cells via inducing apoptosis.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Autoantibodies
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DNA
/
Apoptosis
/
Cell Line, Tumor
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Allergy and Immunology
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Fibrosarcoma
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Immune Sera
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Mice, Inbred BALB C
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Mice, Inbred DBA
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2005
Document type:
Article