Effect of ginkgo biloba extract and dipyridamole on transcription and translation of inducible NO synthbase in rabbits after myocardial ischemia-reperfusion injury / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 240-243, 2006.
Article
in Zh
| WPRIM
| ID: wpr-331711
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Egb761, an extract of ginkgo biloba , and dipyridamole on inducible NO synthase (iNOS) in rabbits after myocardial ischemia-reperfusion injury.</p><p><b>METHODS</b>After being established into ischemia-reperfusion injury model, 35 rabbits were divided randomly into 5 groups: Group A (the sham group), Group B (the model group), Group C (treated with dipyridamole 0.8 mg/kg), Group D (treated with Egb761, 40 mg/kg), and Group E (treated with Egb761 40 mg/kg combined with dipyridamole 0.8 mg/kg), all the medications were administered by intravenous injection 30 min after reperfusion. After administration, myocardial iNOS mRNA expression was detected by RT-PCR and western blot.</p><p><b>RESULTS</b>Myocardial iNOS mRNA transcriptive expression in the 5 groups were A 0, B 157.11 +/- 17.73, C 202.6 +/- 21.84, D 356.13 +/- 24.18 and E 562.34 +/- 35.19 respectively, showing significant difference between the treated groups and group B (P <0.01). The translative expression of myocardial iNOS in the 5 groups were A 34.24 +/- 15.78, B 75.70 +/- 13.71, C 116.89 +/- 22.57, D 143.75 +/- 16.05 and E 195.09 +/- 22.25 respectively, showing significant difference between the treated groups and group B as well (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>Both Egb761 and dipyridamole could increase myocardial iNOS expression in transcriptive and translative levels in rabbits after myocardial ischemia-reperfusion injury, and the combined treatment of them shows a more significant effect.</p>
Full text:
1
Database:
WPRIM
Main subject:
Transcription, Genetic
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RNA, Messenger
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Drugs, Chinese Herbal
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Myocardial Reperfusion Injury
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Random Allocation
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Ginkgo biloba
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Therapeutic Uses
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Dipyridamole
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Drug Therapy
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Nitric Oxide Synthase Type II
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2006
Document type:
Article