Screening and cloning gene of a hepatocyte protein interacting with HCV NS5ATP4A protein / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 286-288, 2008.
Article
in Zh
| WPRIM
| ID: wpr-332255
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen and clone hepatocyte protein interacting with hepatitis C virus NS5ATP4A protein for studying its biological functions.</p><p><b>METHODS</b>Bait plasmids of hepatitis C virus NS5ATP4A were constructed. After verifying that hepatitis C virus NS5ATP4A protein could be steadily expressed in AH109 yeast strain, yeast-two hybrid assay was performed by mating AH109 with Y187 which pre-transformed with liver cDNA library plasmids pACT2, and the diploidy yeast cells were plated on quadruple dropout (QDO) medium and assayed for X-a-gal activity. Nineteen yeast colonies which grew on QDO and had a-gal activity were obtained, and then the library plasmids were extracted and sequenced.</p><p><b>RESULTS</b>Seven genes were screened out and one of them was a formerly unknown gene. They were associated with RNA synthesis, protein translation, cell cycling and tumor immunity.</p><p><b>CONCLUSION</b>NS5ATP4A binding proteins were successfully screened, which offers new clues for further studying the signal transduction pathway of NS5ATP4A and the pathogenic mechanism of HCV.</p>
Full text:
1
Database:
WPRIM
Main subject:
Protein Binding
/
Molecular Sequence Data
/
Base Sequence
/
Gene Library
/
Genome, Viral
/
Cloning, Molecular
/
Viral Nonstructural Proteins
/
Sequence Homology
/
Hepacivirus
/
Two-Hybrid System Techniques
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2008
Document type:
Article