Probability of high resolution full match for human leukocyte antigen loci in unrelated donors and recipients with low resolution match / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1617-1620, 2010.
Article
in Zh
| WPRIM
| ID: wpr-332307
Responsible library:
WPRO
ABSTRACT
This study was aimed to analyze the possibility of high resolution matching for human leukocyte antigen (HLA) loci in unrelated donor-recipient pair with low resolution match in HLA-A, -B, -DRB1 loci. Samples were genotyped for HLA-A, -B, -C, -DRB1 and -DQB1 by polymerase chain reaction sequence based typing (PCR-SBT). The results showed that the total number of patients and the donors were 166 and 274. 97 (58.43%) patients were matched for 1 donor and 47 (28.31%) patients were matched for 2 donors at low resolution level; among 274 donor-recipient pairs, HLA-A, -B, -C, -DRB1 and -DQB1 loci matching for 6/10, 7/10, 8/10, 9/10 and 10/10 were 32 (11.68%), 54 (19.71%), 62 (22.63%), 49 (17.88%) and 48 (17.52%) respectively; there were mismatch in HLA-A, -B, -C, -DRB1 and -DQB1 loci, and the most mismatch was in HLA-C locus. The number of alleles of HLA-A, -B, -C, -DRB1 and -DQB1 loci were 23, 46, 21, 30 and 17 respectively in the donors. The alleles number HLA-A, -B, -C, -DRB1 and -DQB1 loci were 20, 40, 22, 29 and 16 respectively in the patients; the haplotype number of HLA loci were 311 in the donors and 224 in the patients. The high frequency of haplotype was A*02:07-B*46:01-C*01:02-DRB1*09:01:02-DQB1*03:03 (5.63% and 6.88%). It is concluded that the probability of high resolution mismatch of HLA loci is high in unrelated donor-recipient pairs with low resolution match in HLA-A, -B, -DRB1 loci.
Full text:
1
Database:
WPRIM
Main subject:
Tissue Donors
/
Haplotypes
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Histocompatibility Testing
/
HLA-A Antigens
/
HLA-B Antigens
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HLA-C Antigens
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HLA-DQ Antigens
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HLA-DR Antigens
/
Probability
/
Hematopoietic Stem Cell Transplantation
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2010
Document type:
Article