Effect of BCL11A gene on transcription of γ-globin gene / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 628-632, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-332723
Responsible library:
WPRO
ABSTRACT
This study was aimed to explore the effect of BCL11A gene on transcription of γ-globin gene in K562 cells. B-cell lymphoma/leukemia 11A (BCL11A) gene was silenced by small interfering RNA (siRNA) expression vectors in K562 cells (human erythroblastic leukemia cell line). Gamma-globin mRNA level in K562 cells was determined by RT-PCR. Association between the BCL11A gene and γ-globin gene transcription was explored by comparison of mRNA levels. The results indicated that the silence rate of the BCL11A gene in K562 cells by 4 siRNA expression vectors was 49.7%, 55.4%, 78.2%, and 84.1%, respectively. The siRNA expression vector with 84.1% silence rate was transfected into K562 cells, transcription level of γ-globin mRNA in K562 cells transfected with siRNA expression vector increased 2.4 times as compared with control K562 cells. It is concluded that level of γ-globin mRNA increases when the BCL11A gene is silenced. It indicates that the BCL11A gene may be a negative regulator for γ-globin gene expression.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
Nuclear Proteins
/
Transfection
/
Carrier Proteins
/
Gene Expression Regulation, Leukemic
/
Genes, Regulator
/
K562 Cells
/
RNA, Small Interfering
/
RNA Interference
/
Gamma-Globins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2013
Document type:
Article