Mitochondrial mechanisms of apoptosis of human leukemia K562 cells induced by AVVC-1 / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 591-595, 2013.
Article
in Zh
| WPRIM
| ID: wpr-332730
Responsible library:
WPRO
ABSTRACT
This study was purpose to investigate apoptosis pathway of leukemia K562 cells induced by anticoagulant fraction from Agkistrodon acutus venom (AVVC-1). The mitochondrial transmembrane potential (ΔΨm) of leukemia K562 cells was detected by flow cytometry with JC-1 single staining. The expression of cytochrome C in the mitochondrial of leukemia K562 cells was analyzed by Western blot after AVVC-1 treatment. The distribution of cytochrome C in leukemia K562 cells was measured by immuno-fluorescence test. The results showed that the potential of mitochondrial membrane decreased after treatment with different concentrations of AVVC-1 (12.5, 25, 50, 100 µg/ml) for 6 h (P < 0.01). The expression level of cytochrome C protein in mitochondria obviously declined after treatment with 30 µg/ml AVVC-1 for 48 h, and the fluorescent intensity of cytochrome C in cytosol was enhanced at the same time. It is concluded that AVVC-1-induced K562 cell apoptosis is related with mitochondrial damage, and cytochrome C may be a useful agent for investigating human leukemia therapy by using AVVC-1.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Snake Venoms
/
Apoptosis
/
Agkistrodon
/
K562 Cells
/
Cytochromes c
/
Membrane Potential, Mitochondrial
/
Metabolism
/
Mitochondria
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2013
Document type:
Article