Influence of TIEG1 on apoptosis of HL-60 cells and expression of Bcl-2/Bax / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 587-590, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-332731
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate the influence of TIEG1 on apoptosis of HL-60 cells and the expression of Bcl-2/Bax. Different concentration of TIEG1 were used to treat HL-60 cells, the cell growth inhibition rate was detected by MTT method. After treating HL-60 cells with 12.03 ng/ml TIEG1, cell apoptosis was detected with flow cytometry. Bcl-2 and Bax was detected with RT-PCR. The results showed that TIEG1 had inhibitory effect on HL-60 cell proliferation, and in time-and dose-dependent manners. The more obvious inhibitory effect was observed in HL-60 cells treated with TIEG1 of 12.03 ng/ml. During the course of cell apoptosis, Bax expression increased, but Bcl-2 expression decreased (P < 0.05). It is concluded that TIEG1 inhibits HL-60 cell proliferation and induces apoptosis in time and dose-dependent manners. During the course of HL-60 cells apoptosis induced by TIEG1, Bcl-2/Bax are associated with HL-60 cell apoptosis induced by TIEG1.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Gene Expression Regulation, Leukemic
/
Apoptosis
/
HL-60 Cells
/
Proto-Oncogene Proteins c-bcl-2
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Cell Proliferation
/
Bcl-2-Associated X Protein
/
Early Growth Response Transcription Factors
/
Kruppel-Like Transcription Factors
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2013
Document type:
Article