Clone expression and purification of influenza A virus nucleoprotein gene / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To clone the influenza A virus NP gene into expression vector and to purify the target protein, which was used to study the preparation of monoclonal antibody.</p><p><b>METHODS</b>The RNA of influenza A virus was extracted and primers were designed according the NP gene sequence, then the NP gene of influenza A virus was expressed in E.coli DH5alpha and the NP protein was purified by affinity chromatography.</p><p><b>RESULTS</b>The recombinant expression vector-pGEX-4T-2-NP was successfully constructed and relatively pure target protein was obtained.</p><p><b>CONCLUSION</b>Through reasonably controlling the fermentation time, growth temperature and induction concentration, satisfactory soluble target product was obtained.</p>