Genomic sequence of hepatitis A virus L-A-1 vaccine strain / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 360-362, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-333095
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the genome sequence of hepatitis A virus L-A-1 strain which has been applied for live attenuated vaccine production in China, to compare with other HAV strains, to understand some characteristics of L-A-1 strain, and to find the mechanism of attenuation and cell adaptation.</p><p><b>METHODS</b>Genome fragments were prepared by antigen-capture PCR from infected cell (2BS), PCR products were cloned into T vector, sequenced and analyzed by using bioinformatics program.</p><p><b>RESULTS</b>Analysis of the genomic sequences(nt 25-7,418) showed that the open reading frame contains 6,675 nucleotides in length encoding 2,225 amino acids. Sequence homology comparison showed 98.00% and 94.00% homology at nucleotide level, and 98.51% and 98.65% homology at amino acid level with international strains MBB and HM 175, respectively. Through comparison with other attenuated, cell adapted and cytopathic effect (CPE) strains, L-A-1 strain had mutation at nt 152, 591, 646, 687 and insertion at nt 180-181 in 5?NTR and had mutation at nt 3,889 (aa 1 052-Val) in 2B region, these mutations and insertion are molecular basis for cell adaptation; mutation at nt 4,185 (aa 1 152-Lys) in 2C region should be attenuated marker; deletion in 3A region (nt 5,020-5,025) that caused two amino acids deletion is virus fast growth basis.</p><p><b>CONCLUSION</b>Through analyzing L-A-1 strain genomic sequence, certain sites related to cell adaptation and attenuation were found.</p>
Full text:
Available
Health context:
SDG3 - Health and Well-Being
Health problem:
Target 3.3: End transmission of communicable diseases
Database:
WPRIM (Western Pacific)
Main subject:
Adaptation, Biological
/
Vaccines, Attenuated
/
Base Sequence
/
Open Reading Frames
/
Amino Acid Sequence
/
Genome, Viral
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Sequence Homology
/
Gene Deletion
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Hepatitis A Vaccines
/
Hepatitis A virus
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2004
Document type:
Article