Expression of activins, follistatin mRNA in the development of hepatic fibrosis / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 85-88, 2002.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-334308
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the expression changes of activin beta A, beta C, beta E and follistatin mRNA in the development of rat hepatic fibrosis induced by carbon tetrachloride (CCl(4)).</p><p><b>METHODS</b>Hepatic fibrosis was induced in rats by subcutaneous injections of 40% carbon tetrachloride oily solution for a period of 1 to 7 weeks. After carbon tetrachloride injection of 1, 2, 3, 4, 5, 6, and 7 weeks, the 6-12 rats were killed every time. The kinetics of activin beta A, beta C, beta E and follistatin mRNA expression were assessed by the semi-quantity RT-PCR.</p><p><b>RESULTS</b>Activin beta A, beta C, beta E and follistatin mRNA could be detected in normal rat livers. After CCl(4) injection for 2 or 3 weeks, beta A mRNA was transiently decreased and became undetectable, then increased gradually. After CCl injection for 6 and 7 weeks, beta A mRNA level was significantly higher than controls (P<0.01). beta C mRNA could be detected after CCl(4) injection for 1 to 4 weeks and was significantly increased after 5 weeks over controls (P<0.05). beta E mRNA could not be detected after CCl(4) injection for 1 to 5 weeks, but significantly increased after CCl(4) injection for 6 or 7 weeks compared with controls (P<0.01). Except for normal rat liver, no follistatin mRNA was detected in rats after CCl(4) injection.</p><p><b>CONCLUSIONS</b>Activins and follistatin have different expression changes in the development of hepatic fibrosis and the imbalance of activins and follistatin expression may involve in the formation of hepatic fibrosis.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
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Carbon Tetrachloride
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Gene Expression
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
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Activins
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Inhibin-beta Subunits
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Follistatin
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Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2002
Document type:
Article