Construction of a bait plasmid containing HBV PreS1 gene in a yeast two-hybrid system and evaluation of its toxicity and self-activation / 南方医科大学学报
Journal of Southern Medical University
; (12): 1955-1959, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-336047
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct a yeast expression vector of hepatitis B virus (HBV) PreS1 gene using the Sos-recruitment system (SRS), and evaluate the effect of the expression product on the growth of the yeast cells and activation of the reporter gene.</p><p><b>METHODS</b>The coding sequence of HBV preS1 was amplified by PCR and cloned into the yeast expression plasmid pSos. The recombinant bait plasmid pSos- PreS1 was verified by sequencing before transformation into competent yeast cells. The effects of the expression product on the yeast cell growth and activation of the reporter gene were evaluated.</p><p><b>RESULTS</b>The yeast expression vector of HBV PreS1 gene was constructed successfully. The recombinant bait plasmid showed no toxic effect on yeast cdc25H cells without a self-activation of the reporter gene.</p><p><b>CONCLUSION</b>The SRS can be used to study the proteins interacting with HBV PreS1 protein and provides a means for obtaining insight into the pathogenic mechanism of HBV.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Protein Precursors
/
Receptors, Virus
/
Yeasts
/
Recombinant Fusion Proteins
/
Cloning, Molecular
/
Two-Hybrid System Techniques
/
Genetic Vectors
/
Genetics
/
Hepatitis B Surface Antigens
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Document type:
Article