Effects of survivin antisense RNA and HSP70 double gene transfection on MCF-7 cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 1587-1589, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-336135
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of survivin antisense RNA and HSP70 double gene transfection on breast cancer cell line MCF-7.</p><p><b>METHODS</b>MCF-7 cells was transfected with the double-gene vector pIRES2-EGFP-survivin antisense RNA/HSP70 via liposome. After a 72-h transfection, the cells were collected for observation under inverted fluorescent microscope. The changes of survivin mRNA and HSP70 protein expressions in the cells were detected with real-time PCR and Western-blot before and after the cell transfection, and the apoptotic rate of the transfected MCF-7 cells was detected by flow cytometry analysis with Annexin-V-cy5/7AAD double staining.</p><p><b>RESULTS</b>Green fluorescence was detected in MCF-7 cells transfected with the double-gene expression vector and the empty vector under inverted fluorescent microscope. The expression level of survivin mRNA in the cells was reduced effectively after the transfection with the double-gene expression vector, which also induced obvious cell apoptosis and enhanced the expression level of HSP70 protein as compared with those in MCF-7 cells transfected with the empty vector and the untransfected MCF-7 cells.</p><p><b>CONCLUSION</b>Survivin antisense RNA can interfere with the expression of endogenous survivin and induce apoptosis of MCF-7 cells. HSP70 can increase the expression of HSP70 protein in MCF-7 cells.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Transfection
/
RNA, Antisense
/
Apoptosis
/
HSP70 Heat-Shock Proteins
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Inhibitor of Apoptosis Proteins
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MCF-7 Cells
/
Genetics
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Document type:
Article