Site-directed mutagenesis of MSTN gene by gene targeting in Qinchuan cattle / 生物工程学报
Chinese Journal of Biotechnology
; (12): 410-416, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-336211
Responsible library:
WPRO
ABSTRACT
Myostatin, a member of the transforming growth factor beta (TGF-beta) family, is a negative regulator for muscle growth. Loss of the function of this gene is associated with the phenotype described as "double muscling", an extreme form of muscle development characterized by a large increase in muscle mass. Two replacement vectors, pA2T-Mstn4.0 and pA2T-Mstn3.2, were constructed, linearized, and transfected into the bovine fetal fibroblasts through electroporation. 170 drug-resistant cell colonies were obtained in cell culture medium containing 600 microg/mL G418 and 50 nmol/L GCV. Targeted homologous integration occurred in colony No. 58 as identified by PCR, and the targeted colony was further confirmed by sequencing and Southern blotting. This suggested that one allele of myostatin was successfully mutagenized in bovine fetal fibroblasts.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
China
/
Mutagenesis, Site-Directed
/
Electroporation
/
Gene Targeting
/
Cell Biology
/
Myostatin
/
Fetus
/
Fibroblasts
Type of study:
Prognostic study
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Document type:
Article