Expression and localization of the nuclear factor-κB classical signaling pathway in the radial artery of diabetic patients / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the expression and localization of the nuclear factor-κB (NF-κB) classic signaling pathway in the radial artery of the coronary artery bypass grafting (CABG) patients with diabetes and non-diabetes.</p><p><b>METHODS</b>Samples of radial artery from 36 cases of diabetic and non-diabetic patients were randomly collected from January 2012 to December 2012.In the diabetic group there were 13 male cases, 5 female cases, with an average age of (69 ± 6) years.In non-diabetes group there were 13 male cases, 5 female cases, with an average age of (70 ± 6) years.HE staining techniques was used to test the morphology of radial artery. Immunohistochemical techniques was then used to test the expression and localization of key factors (inhibitor of kappa B kinase β (IKKβ), P50, P65, tumor necrosis factor-α (TNF-α) and endothelial nitric oxide synthase (eNOS)) in the NF-κB classical signaling pathway in the two groups of patients.</p><p><b>RESULTS</b>The radial artery intima was signifiantly thickened in the diabetic patients when compared with non-diabetic patients by HE staining. And there wss a lot of inflammation and foam cell infiltration in the radial artery intima of the diabetic patients. Using immunohistochemical techniques the expression of IKKβ, P50, P65 in diabetic group were obviously higher than non-diabetic group (4.4 ± 0.5 vs. 1.1 ± 0.6, 4.8 ± 0.7 vs. 1.2 ± 0.7, 4.2 ± 0.4 vs. 1.6 ± 0.7, t = 18.238, 15.052, 13.535, all P < 0.01).In the aspect of inflammatory cytokines, the expression of TNF-α and eNOS factors in diabeics group were significantly higher than non-diabetic group (5.5 ± 0.5 vs.1.4 ± 0.7, 3.3 ± 0.5 vs. 0.8 ± 0.4, t = 20.118, 16.764, all P < 0.01) also. And the five kinds of protein were mainly located in radial artery intima layer cells in diabetic patients.</p><p><b>CONCLUSIONS</b>Expression levels of 5 key factors in NF-κB classic signaling pathway are significantly higher in radial artery intimal layer of diabetic patients than non-diabetic patient's. And Positive signals are more concentrated in the radial artery intima layer of cells.</p>