Establishment of reverse-phase high-performance liquid chromatography with chiral reagent derivatization for separation of fexofenadine enantiomers / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 155-159, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-336726
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a precolumn chiral derivatization method for determination of fexofenadine enantiomers, a chiral substrate of OATP1B1, in cellular model.</p><p><b>METHODS</b>R-(+)-phenylethyl isocyanate was selected as chiral derivatization reagent, which was reacted with fexofenadine to form carbamate derivatives. Enantiomers were identified by LC/MS and separated by RP-HPLC.</p><p><b>RESULTS</b>Under the experimental conditions, the fexofenadine enantiomers were separated completely. The standard curve was linear over the concentration range of 25-100 ng/ml (R(2)=0.9992, 0.9989). Accuracy was 101.1% and 98.3%, intra-precision was 2.4% and 3.1%, inter-precision was 3.1% and 4.0% for D1 and D2, respectively.</p><p><b>CONCLUSION</b>The method established is sensitive and accurate for determination of fexofenadine enantiomers in cells.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Stereoisomerism
/
Chromatography, High Pressure Liquid
/
Terfenadine
/
Methods
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2014
Document type:
Article