Development of MTS/pms colorimetric assay in the proliferation of leukemic cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 438-440, 2002.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-337651
Responsible library:
WPRO
ABSTRACT
In order to establish a new more rapid, safe and sensitive colorimetric assay for the proliferation of leukemic cells, MTS/pms has been developed. This automated colorimetric assay is based on the characteristic of viable and metabolically active leukemic cells to cleave MTS/pms into a water-soluble product whose optical density is determined at 492 nm by an automated microtiter-plate reader photometer. The results indicated that only active leukemic cells cleaved MTS/pms into product measured, and dead cells did not reduce MTS/pms. A linear relations hip were found between the viable cell number and optical density of MTS/pms cleaved by HL-60 and K562 cell (r = 0.963). Compared with MTT and INT assays, the reduced product of MTS/pms is water-soluble. It is concluded that MTS/pms colorimetric assay is more rapid, accurate and sensitive for the bioassay of proliferation of leukemic cells.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Tetrazolium Salts
/
Thiazoles
/
Leukemia
/
Cell Division
/
Colorimetry
/
HL-60 Cells
/
K562 Cells
/
Formazans
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2002
Document type:
Article